Diagnostic ability of miR-19a and Let-7f along with platelet and leukocyte extracellular vesicles in acute ischemic stroke patients

Abstract Background Ischemic stroke (IS) is one of the most frequent causes death. Since miRNAs have been illustrated to play an important role in various processes through regulation multiple genes, and platelet function, their utility as novel biomarkers should be determined. Purpose We aimed analyze circulating platelet-derived miR-19a-3p, miR-186-5p, Let-7f, platelet-extracellular vesicles (EVs), leukocyte-EVs, endothelial-EVs levels 24-h 7-days after IS diagnostic prognostic/predictive 28 acute 35 control patients. Methods Blood samples patients diagnosed with hyper reactivity were collected age- gender-matched individuals free risk factors for cardiovascular disease. Platelet was assessed by AA-, TRAP-, ADP-induced aggregometry. PlasmaRNA extracted; quality RNA assessed: fluorometric assay; RT-PCR expression measurement flow-cytometry EVs determination p<0.05. Results Patients on day-1 had significantly higher AA-induced aggregometry compared controls (p=0.001). normal activation miR-186-5p HPR at acute-stroke (p=0.034). Seven days stroke, decreased same (p=0.036). elevated platelet-EVs (CD62) concentration day 1 (p=0.012). Similarly, leukocyte-EVs (CD45) function (p=0.002). Diagnostic values baseline evaluated receiver operating characteristic curve analysis. ROC showed that pooling miR-19a-3p expressions, platelet-EVs, yielded a AUC than value each individual biomarker 0.893 (95% CI, 0.79–0.99). moderate minor IS. analysis 0.867, 0.74–0.10) p=0.001. Conclusions Our alteration Combination might can predict severity Funding Acknowledgement Type funding sources: Public grant(s) – National budget only. Main source(s): Science Centre - Narodowe Centrum Nauki